Generator

Part:BBa_K624055

Designed by: Yi-Hong Liou   Group: iGEM11_NYMU-Taipei   (2011-10-05)

pYMB essentials + RBS(Pmsp3) + tetR + RBS(Pmsp3) + GFP

Pmsp1 + RBS(Pmsp3) + tetR + RBS(Pmsp3) + GFP

The Pmsp1 is the second strongest promoter on AMB-1.

RBS(Pmsp3) is the RBS site from Pmsp3, the strongest promoter of AMB-1.(BBa_K624012)

TetR serves as a switch, which can turn on the downstream gene when tetracycline is present; and vice versa.

GFP serves as a reporter to show if the construct work or not.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 898
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2561


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Categories
Parameters
None